Colocalization of 5HTR_B- and PTTH-ir in the erly pupal BR-SOG of

<p><b><i>A. pernyi</i></b>. <i>Bm</i>PTTH-ir/<i>Ap</i>PTTH-ir in the 5-day-old pupal brain of <i>A. pernyi</i> and its colocalization with <i>Ap</i>5HTR_B-ir (red filled circles). <i>Bm</i>EH-ir in the 5-day-old pupa brain and its colocalization with <i>Ap</i>5HTR_B-ir (blue filled circles) and unique distribution (open circles) of <i>Bm</i>EH-ir in other regions of the brain. (A) The loaction of detected cells. Lower-case letters correspond to the regions shown in the photographs (e.g., b to B). (B) One <i>Bm</i>EH-ir neuron in the PI. (C) <i>Bm</i>EH-ir in the DC region. (D, H) 5HTR_B-ir in the DL region. (E) <i>Bm</i>PTTH-ir in the DL region. (F) Merged image of <i>Bm</i>PTTH-ir and 5HTR_B-ir in the DL region. (G) <i>Ap</i>PTTH-ir in the DL region. (I) Merged image of <i>Ap</i>PTTH-ir and 5HTR_B-ir in the DL region. (J) 5HTR_B-ir in the DC region. (K) One EH-ir neuron in the DC. (L) Merged image of <i>Bm</i>EH-ir and 5HTR_B-ir in the DC region. Scale bar = 100 µm.