Characterization of the lateral migratory stream (LMS) of newly born neurons and identification of immature neurons in piglet forebrain.

<p><b>A</b>. Confocal image showing the colocalization of BrdU (red) and NeuN (green) in subsets of neurons in the piriform cortex. Single-labeled neurons are green, single-labeled BrdU⁺ nuclei are red, and double-labeled neurons are yellow. <b>B</b>. Immunofluorescence showing the colocalization of BrdU (red) and doublecortin (Dcx, green) in the LMS. One Brdu⁺ cell (top cell) does not express Dcx, but another Brdu⁺ cell (bottom cell) is Dcx⁺ as seen by the yellow around the nucleus and the trailing Dcx-labeled process (arrow). <b>C</b>. Immunofluorescence showing the colocalization of BrdU (red) and β-tubulin III (βTubIII, green) in the LMS. <b>D</b>. Immmunoperoxidase staining for β-tubulin III (βTubIII) revealed a column of βTubIII⁺ immature neurons (brown cells) below the anterior striatum within the LMS. Inset shows a βTubIII⁺ LMS neuron with a fusiform morphology and leading and trailing processes. <b>E</b>. In piriform cortex, βTubIII⁺ immature neurons were found in cellular islands in layer II (arrow). Boxed area is shown at right at higher magnification and rotated 90°. <b>F</b>. In OB, βTubIII⁺ immature neurons (arrows) were found in the granule cell layer (GCL). These cells possessed extensive local vertical-oriented arborizations (inset) consistent with an interneuron morphology [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0081105#B46" target="_blank">46</a>]. EPL, external plexiform layer; MCL, mitral cell layer. Scale bars = 25 µm (A), 5 µm (B), 4 µm (C), 25 µm (D), 8 µm (D inset), 32 µm (E), 12.5 µm (E right), 100 µm (F), 25 µm (F inset).