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Details of verification using amplicon-based sequencing on the Ion Torrent.

Publication date

November 2013

DOI

10.1371/journal.pone.0074380.t004

Abstract

<p>Details of verification using amplicon-based sequencing on the Ion Torrent.</p

Extracted data

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RESTseq - example of a library sequenced on a Ion Torrent - GC content across sequenced fragments - note the first 3 basepairs are CGA

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<p>RESTseq - example of a library sequenced on a Ion Torrent - GC content across sequenced fragments...

Fusion oligonucleotide primers containing target specific sequences (White, Bruns et al., 1990, Issakainen, Jalava et al., 1999; emboldened), sequencing adapters (plain text) and the Ion Torrent key sequence (underlined).

Daniel P. Tonge (550715)
Catherine H. Pashley (550716)
Timothy W. Gant (27460)

April 2014

<p>Fusion oligonucleotide primers containing target specific sequences (White, Bruns et al., 1990, I...

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Wielstra, Ben
Vörös, Judit
Arntzen, Jan W.

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Curtis Kapsak (833607)
Kevin Libuit (799859)
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