Voltage-gated Ca²⁺ channels are not affected by NOC7.

<p>Ca²⁺ currents were recorded in whole-cell voltage-clamp mode. Voltage steps from a holding potential of −60 mV to +60 mV were applied in 10 mV increments. A: Representative traces of Ca²⁺ currents evoked by a voltage step from −60 mV to +10 mV before (upper), during the initial phase (middle), and during the plateau phase of treatment with NOC7 (100 µM, lower). B: Representative I–V plot of Ca²⁺ current measured at the peak amplitude and expressed as normalized Ca²⁺ current (pA/pF) before and after NOC7 application. Note that NOC7 did not have an obvious effect on Ca²⁺ currents. C: Quantification of the effect of NO on Ca²⁺ currents showing that treatment with NOC7 did not have a significant effect on normalized peak currents compared to control groups during both initial and plateau phases. Subsequent application of the Ca²⁺ channel blocker CdCl₂ (100 µM) fully eliminated Ca²⁺ currents.