NO causes membrane potential depolarization in B19 neurons.

<p>A: A silent B19 neuron was depolarized and started firing after treatment with NOC7 (100 µM, bath application; gray bar). Note electrical recording artifact upon drug addition. Enlarged areas of interest below the main recording trace (marked by black dashed boxed) show details of the recording (note that APs are clipped to emphasize membrane depolarization). The initial depolarization to NOC7 at 30 s was stronger than that during the plateau phase at 3 min. B: Representative data showing membrane potential depolarization of B19 neurons by another NO donor, DEA/NO (100 µM). C: Quantification of the changes in membrane potential during the initial phase such as shown in A and B. Both NOC7 and DEA/NO caused a significant depolarization compared to that of their vehicle control groups. D. Quantification of the plateau depolarization showing that both NOC7 and DEA/NO caused significant depolarization during the plateau phase.