Motor neurons in brainstem resist depolarization in response to OGD or ouabain.

<p>A) Typical response of a DMV neuron to current pulse injection. When depolarized from negative holding potentials, DMV neurons display characteristic delayed firing (asterisk) as well as a delay in return to baseline(arrow) following a hyperpolarizing current step. B) During OGD, a DMV neuron initially hyperpolarizes before depolarizing with slow spike inactivation to −5 millivolts. Back in control aCSF the neuron repolarizes. C) DMV neuron response to 10 minutes of ouabain exposure. The neuron gradually depolarizes to −10 mV. Upon return to control aCSF the neuron starts repolarizing but the recording is lost (not shown).