Expression of IFN1 and IFNAR variants in RTG-2 cells.

<p>(A) Constitutive expression in normal cells; (B, C, D) expression modulation of genes in RTG-2 cells incubated (B) or transfected (c) with polyI∶C, or in head kidney tissue of fish (n = 4) after VHSV infection (D). Gene expression was analysed by real time PCR and normalised to EF-1α. Fold change of gene expression was calculated by comparing the normalised gene expression between the treated and untreated groups. * = P<0.05 relative to unstimulated/uninfected control samples at the same time point.