DUSP1 is involved in steps occurring before mature virion formation.

<p><b>A</b>) Confocal microscopy of DUSP1 WT and KO cells. Cells were infected at 5 PFU/cell with either WR, MVA or NYVAC. Samples were fixed at 5 h 30 min and stained with antibodies against viral proteins A14 or A17 (green), or probes that detect phalloidin (red) and DNA (DAPI, blue). Black panels show representative images of WT and KO cells infected with MVA. <b>B</b>) Western-blot analysis of DUSP1 WT and KO cells infected at 5 PFU/cell with either WR, MVA or NYVAC. Samples were collected at 2, 6, 16 and 24 hours post-infection and analyzed by Western-blot with antibodies against viral proteins A10, L4 and A17 and cellular protein tubulin. <b>C</b>) Confocal microscopy of DUSP1 WT and KO cells infected with MVA at 5 PFU/cell and fixed at 16 hpi. Lower panels represent magnifications (5×) of the pictures above. Samples were stained with antibodies against viral protein A27 (green), phalloidin (actin, red) and DNA (DAPI, blue).