DUSP1 suppression increases STAT1 activity and nuclear translocation.

<p>Phosphorylation (A) and nuclear translocation (B) of STAT1 were assessed by cell-based ELISA and confocal microscopy, respectively. (A) Expression of phospho-STAT1 was normalized to that of STAT1 and then to the ratio in LV-cont-infected cells. (B) Red, anti-STAT1; blue, DAPI. Merged image allows assessment of nuclear localization of STAT1; original magnification ×200. All data are representative of at least three independent experiments.