EAF2-deficiency induces murine prostatic intraepithelial neoplasia (mPIN) and stromal defects in C57BL/6J and FVB/NJ mice at age 20–24 mos.

<p>A. Strain-specific incidence rate of mPIN in EAF2^−/− mice at age 20–24 mos compared to 0% incidence in wild-type (WT) controls (*p<0.01). B. Strain-specific incidence in stromal defects in EAF2^−/− mice at age 20–24 mos compared to 0% incidence in WT controls (*p<0.01). Number of animals in each group is indicated in parenthesis. C. EAF2^−/− mice on a 129P2/OLA-C57BL/6J background developed mPIN lesions but not stromal defects (right panel inset, black arrow designating mPIN). D. Aged EAF2^−/− mice on a C57BL/6J background displayed mPIN and stromal hypertrophy (right panel, solid black arrow) and interstitial fibrosis (right panel inset, dashed arrow) at age 20–24 mos compared to WT controls. E. Aged EAF2^−/− mice on an FVB/NJ background displayed mPIN (right panel inset, solid black arrow) and increased stromal inflammation and fibrosis (right panel inset, dashed black arrow) compared to WT controls. F. Prostate stromal cells surrounding mPIN lesions in aged EAF2^−/− mice displayed immunopositivity for reactive stroma marker tenascin-C (TNC). Original magnification 10X, inset 40X. Scale bars indicate 200 micron in 10X, 50 micron in 40X. All images are ventral prostate lobe.