mPIN lesions in 10-month-old untreated mice.

<p>To determine the development of mPIN lesions over time without hormone treatment, 10-month-old untreated mice of each genotype were sacrificed and prostate tissue was obtained. H&E staining of prostate tissue was used for mPIN grading, applying the Bar Harbor grading system, and subdivided into mPIN I-IV as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060277#pone.0060277.s006" target="_blank">Table S6</a>. <b>A</b>) Representative picture of the maximum grade reached: wt) grade 6; tgPim1) grade 5; PTEN-Het) grade 13; tgPim1/PTEN-Het) grade 11. <b>B</b>) Incidence (in %) of mPIN lesions per genotype in 10-month-old mice. Percentage of developed mPIN grade (mPIN I-IV and microinvasive carcinoma) was determined for each genotype using H&E staining of prostate tissue. <b>C</b>) mPIN lesions in 10-month-old untreated mice. The graphs represent the grading observed in the different prostates analyzed; statistical relevance is also shown: * = <0.05; ** = <0.01; *** = <0.001. <b>D:</b> H&E staining of a microcarcinoma. Microcarcinoma in a 10-month-old PTEN-Het mouse.