Hyperlocomotion of <i>Elfn1^−/−</i> mice.

<p>(A–E) <b>Experiment 1.</b> (A) The number of crosses on day 1 of open field testing was significantly higher for <i>Elfn1^−/−</i> mice than in <i>Elfn1^+/−</i> mice (<i>p</i> = 0.01; n = 8/genotype). (B) On day 2, in the same open field, there was no significant difference between the groups (<i>p</i> = 0.138) but the mean number of crosses remained higher for <i>Elfn1^−/−</i> mice (90.13±13.32) versus <i>Elfn1^+/−</i> mice (67.38±5.62; n = 8/genotype). (C) Introduction to a novel environment again confirmed a higher number of crosses in <i>Elfn1^−/−</i> mice versus <i>Elfn1^+/−</i> mice (<i>p</i> = 0.0025; n = 8/genotype). (D) Graph shows the number of crosses before and after 0.5 mg/kg AMPH for each mouse in experiment 1 (n = 8/genotype). <i>Elfn1^+/−</i> (white circles), <i>Elfn1^−/−</i> (black circles). (E) The mean percentage distance travelled by <i>Elfn1^+/−</i> and <i>Elfn1^−/−</i> mice after 0.5 mg/kg. Student's <i>t</i> test <i>p</i> value = 0.0159. (F–K) <b>Experiment 2.</b> (F) On day 1, statistical analysis revealed no significant difference between the groups however, the mean distance travelled by <i>Elfn1^−/−</i> mice was greater than by <i>Elfn1^+/−</i> mice (5701±278.8 for <i>Elfn1^−/−</i> and 5108±222.4 for <i>Elfn1^+/−</i>, n = 10/genotype; <i>p</i> = 0.1141). (H) On day 2, in the same open field, <i>Elfn1^−/−</i> mice travelled a significantly greater distance than <i>Elfn1^+/−</i> mice (p = 0.0381; n = 10/genotype). (G) On day 1, the mean distance travelled by <i>Elfn1^−/−</i> mice remained higher than for <i>Elfn1^+/−</i> mice over the 10 minutes, and a significant difference between the genotypes is evident in the final 2 minute bin (p = 0.0021; n = 10/genotype). (I) On day 2, a significant difference between groups in the mean distance travelled is identified at the 4–6 minute and 8–10 minute time bins (<i>p</i> = 0.0024 and <i>p</i> = 0.0067 respectively). All data expressed as means, and Student's <i>t</i> test used to analyse statistical difference betwen the groups. Post-hoc analysis is not corrected for multiple testing. (J) Graph depicts differences in distance travelled by each mouse when administered saline and 2.0 mg/kg AMPH in experiment 2 (n = 10 <i>Elfn1^+/−</i>, n = 9 <i>Elfn1^−/−</i>). (K) The mean percentage distance travelled by <i>Elfn1^+/−</i> and <i>Elfn1^−/−</i> mice after 2.0 mg/kg of AMPH. Student's <i>t</i> test <i>p</i> value = 0.0029 for 2.0 mg/kg.