Rhein suppresses SHH signaling in-vivo.

<p>(A) The fluorescent images demonstrated the immunoreactivites of GLI1 (green; FITC) and SHH (red; Rhodamine) in paraffin-embedded pancreatic tissues in which nuclei were stained blue with DAPI. (B) Protein levels of GLI1 and SHH in pancreatic homogenates of the mice were visualized on immunoblots probed with anti-GLI1 and anti-SHH antibodies whereas β-ACTIN was served as the loading reference. (C) Integrated densities of the immunobands of SHH and GLI1 were measured. Readings were normalized to the internal reference β-ACTIN and expressed as fold change over control. (D) Transcripts of <i>Gli1</i> and <i>Gli2</i> were amplified by means of qPCR, normalized to the endogenous reference <i>Gapdh</i> and expressed as fold changes over the Control group. * <i>p</i>< 0.05 when comparing to the Control group whereas <i>Φ </i><i>p</i>< 0.05 when comparing to the Cerulein group.