The expression of Shh increased in cerulein-induced pancreatic acinar cell AR42J cells.

<p>(<b>A</b>)<b>.</b> Real time PCR showed the mRNA levels of the <i>Shh, Dhh</i> and <i>Ihh</i> in rat pancreatic acinar cell AR42J cells treated with different concentration of cerulein for 24 hours, and (<b>B</b>) Western blot confirmed the protein expression change, and the <i>Gapdh</i> and β-actin ware served as control respectively. (<b>C</b>). ELISA detected the secreted Shh in the supernatant of AR42J cell cultures treated with different concentration of cerulein for 24 hours. (<b>D</b>)<b>.</b> Real time PCR showed the change of hedgehog signaling components mRNA expression, <i>Shh, Gli1, Ptch1</i>. Concentration of the recombinant Shh N-terminal was 0, 0.5, 1, and 2 µg/ml. <i>Gapdh</i> was used as an endogenous control.