The expression of Shh was upregulated in cerulein-induced mice model of acute pancreatitis in vivo.

<p>(<b>A</b>). Real time PCR showed the mRNA levels of <i>Shh, Dhh</i> and <i>Ihh</i> during the development of cerulein-induced mice model of acute pancreatitis. 2 µg of total RNA isolated from fresh tissue was used for reverse transcription, and 1 µl of cDNA was used for qPCR reaction. The <i>Gapdh</i> was used as the control. (<b>B</b>). RT-PCR (upper two panels) and Western Blot (lower two panels) verified the mRNA expression and protein level of <i>Shh</i>. In the DNA electrophoresis 2% of gel was used, in the Western blot 4–10% gradient gel was used, and the <i>Gapdh</i> and β-actin were served as control respectively. (<b>C</b>). Immunohistochemistry showed the expression of Shh in pancreatic tissue treated with 50 µg of cerulein for time points at 0 hour, 8 hours, 16 hours and 24 hours. Red bar, 20 µm.