Effects of treatment with APAP and (PhSe)₂ on PTP opening in liver mitochondria based on swelling measurements.

<p>(A) The traces are representative of five independent experiments. (B) Means of the five experiments swelling. Mice were given acetaminophen (600 mg/kg, i.p.) and 1 h after were treated with or without (PhSe)₂ (15.6 mg/kg, i.p.), and were killed at 4 h after the APAP treatment. Mitochondria (0.1 mg/ml) were incubated in the reaction medium containing 230 mM Mannitol, 70 mM Sucrose, 1 mM K₂HPO₄, 20 mM Tris-HCl, pH 7.4 and was energized by 5 mM Glutamate and 5 mM Succinate. The light scattering was monitored after adding CaCl₂ (100 µM). Dates are expressed as means ± SEM, (n = 5). Significance was assessed by one-way analysis of variance (ANOVA), followed by Newman-Keuls's test for post hoc comparison. Significant differences are indicated by ^*p<0.05 when compared with control group. Significant difference is indicated by ^#p<0.05 when compared with APAP group.