Role of ATP in cell-death induced inflammation in Acetaminophen-liver toxicity.

<p>(A, B) Myeloperoxidase activity in the liver (A) and serum levels of ALT (B) of mice challenged with i.p. injection of 300 mg/kg of Acetaminophen in C57BL/6 or P2X7R deficient mice. (C, D) Myeloperoxidase activity in the liver (C) and serum levels of ALT (D) of mice challenged with i.p. injection of 300 mg/kg of Acetaminophen in intact or boiled apyrase treated C57BL/6 mice. (E, F) The total neutrophil (E) and monocyte (F) numbers in the peritoneal cavity of WT C57BL/6 mice and P2X7 receptor -deficient mice 16 hours after i.p. injection of liver homogenate. The data are combined results of 2–3 experiments and represented as means ±SEM (<i>n</i> = total number of mice from the multiple experiments for each group). NS, not significant versus WT groups (A, B, E, F). NS, not significant versus boiled apyrase groups (C, D). PBS groups, WT mice received i.p. PBS.