Effects of treatment with APAP and (PhSe)₂ on the mitochondrial membrane potential in liver mitochondria of mice.

<p>(A) The traces are representative of five independent experiments. (B) Means of the five experiments mitochondrial transmembrane electrical potential (Δψ_m). Mice were given acetaminophen (600 mg/kg, i.p.) and 1 h after were treated with or without (PhSe)₂ (15.6 mg/kg, i.p.), and were killed at 4 h after the APAP treatment. Mitochondria (0.5 mg/ml) were incubated in the reaction medium containing 230 mM Mannitol, 70 mM Sucrose, 0.02 mM EDTA, 1 mM K₂HPO₄, 20 mM Tris-HCl, pH 7.4 and was energized by 5 mM Glutamate and 5 mM Succinate. The mitochondria and 2,4 DNP (100 µM) were added where indicated by arrows. Dates are expressed as means ± SEM, (n = 5). Significance was assessed by one-way analysis of variance (ANOVA), followed by Newman-Keuls's test for post hoc comparison. Significant differences are indicated by ^*p<0.05 when compared with control group. Significant difference is indicated by ^#p<0.05 when compared with APAP group.