Histological analysis of hCLS in the liver from MC4R-KO mice fed a WD.

<p>(A) Serial sections of the liver from MC4R-KO mice fed a WD for 20 weeks stained with F4/80, αSMA, and type I collagen antibodies. Immunofluorescent analysis for F4/80 (B-G), type I collagen (B, E), glial fibrillary acidic protein (GFAP) (C, F), and fibroblast specific protein 1 (FSP1) (D, G) in the liver from wildtype and MC4R-KO mice at 20 weeks. (H) Immunofluorescent analysis for F4/80 and CD11c. (I) Immunofluorescent analysis for F4/80 and lipid droplet (BODIPY). The nuclei were counterstained with DAPI (B–I). Scale bars, 50 µm. (J) Electron micrograph of hCLS. Aggregated macrophages around a lipid droplet (arrows). Fibroblasts (*F) and hepatocytes (*H) are detected around hCLS.