Histology and cytokine production in the liver.

<p>(A) A liver section was stained with ORO (frozen section) in SFD- and HFD-fed mice. (B) Red-stained lipid droplets in liver sections of HFD-fed mice were quantified with image analysis software. (C) Serum levels of ALT were quantified with the Drychem system. (D, E) The production of cytokines from HMNC of HFD-fed mice stimulated with LPS for 20 h (n = 3–4 female mice in each group). The results are expressed as mean ± s.d. Statistical analysis was performed according to the Tukey-Kramer test. *<i>p</i><0.05, **<i>p</i><0.01.