OAPs changes within and outside a HIFU lesion.

<p>(A) Photograph and masson's trichrome (MT) stained slide of a wedge preparation after HIFU ablation (top panels) along with the corresponding binary lesion mask (lesion as white and non-lesion as black) (bottom left panel), ΔAPA obtained at <i>t</i> = 32 s. Black dash lines highlight lesion edges. (B) ΔAPA (n = 13) within lesion and outside lesion (non-lesion) determined by lesion mask. A black dash line indicates a threshold (−0.43±0.01) of ΔAPA used to predict irreversible lesions determined from receiver-operating characteristic (ROC) analysis. (C) ROC curves for ΔAPA detection of lesion for all cases (individual and overall curve are marked gray and red respectively) with an area under ROC curve (AUC) of 0.961. Inset: ΔAPA predicted lesion (red) for the representative example in (A) is overlaid on the real binary lesion mask.