Spatiotemporal changes of transmural AP surrounding HIFU lesion.

<p>(A) Photograph of a left ventricle wedge with a HIFU lesion. (B) Masson's trichrome (MT) stained slide corresponding to the same tissue plane in A. Black dash line encircles lesion boundary. Black asterisks labeled representative locations at inside lesion, border, and normal tissue. (C) Microscopically magnified (40X) MT images at labeled locations in B: lesion (a1), border (b1), and normal tissue (c1). Corresponding normalized OAPs (a2, b2, c2) before (blue) and after (pink) HIFU ablation. (D) Amplitude of fractional fluorescence intensity (ΔF) before and 24 s after HIFU ablation, and change of action potential amplitude (ΔAPA) with dashed line highlighting the same lesion in B. (E) APD₅₀ before and after HIFU ablation and ΔAPD₅₀. (F) APD₈₀ before and after HIFU ablation, and ΔAPD₈₀.