WGCNA analysis of miRNA expression in CTX and MB of mice subjected to CIE paradigm identified distinct modules of coexpressed miRNAs.

<p>A and B show dendrograms produced by average linkage hierarchical clustering. Horizontal color bars represent different coexpression modules. Bar sizes correspond to the number of miRNAs in each module. SoftPower β = 10 (A), 9 (B), minModuleSize = 5, cutHeight = 0.995, deepSplit = 2 (see <i>Methods</i>). Tables C, D show the relative contribution of miRNAs to CIE paradigm, in terms of correlation (<i>Corr.</i>) between the individual top 20 coexpressed miRNAs sorted by their gene significance (<i>GS</i>) for the EoC trait, with relative p-values and rank. Module number and color information are also included. Full lists are reported in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082565#pone.0082565.s001" target="_blank">Dataset S1</a>. Tables E-H show correlation between modules of coexpressed miRNAs (E, F) or proteins (G, H) and the EoC trait, or the average 2BC ethanol consumption, as well as individual proteins or miRNAs as traits. Modules are named by a number and a color. Protein names are followed by corresponding gel spot number. In correlation columns, blue represents negative and red represents positive correlations, as reported on the legend. Green p-values are <0.05. Part of the data shown in G and H is taken from our previous study <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0082565#pone.0082565-Gorini2" target="_blank">[46]</a>.