Expression and activity of MMPs.

<p>A & B. Total LV lysates (50 µg), prepared from sham and non-infarct (NINF) and infarct (INF) LV regions, were analyzed by western blot using anti-MMP-2 (A) and anti-MMP-9 (B) antibodies. The upper panels show autoradiograms indicating immunostaining for MMP-2, MMP-9, and GAPDH. The lower panels exhibit quantitative analyses of MMP-2, MMP-9 normalized to GAPDH. *p<0.05 vs sham; ^#p<0.05 vs NINF; ^$p<0.05 vs WT-INF; n=7. C&D. Total LV lysates (50 µg), prepared from the infarct LV regions were analyzed by in-gel zymography. C. MMP-2 activity. D. MMP-9 activity. *p<0.05 vs WT-MI; n=3.