Effect of SIRT1 and FOXO3A on the angiogenic activity of endothelial cells.

<p>A. (1) ECs were treated for four different treatments before infection: 1. resveratrol(20umol) for 4 hours; 2. DMSO;3. SIRT siRNA(100nmol) for 24 hours; 4. negative control. (2) Cells were transfected with SIRT1 siRNA or control siRNA(100 nmol) and then treated with resveratrol(20umol) before infection. Proliferation was measured with CCK-8 assay at 72hpi. B. Cell migration analysis of ECs that were treated with the same methods as those used in Fig. 2A. C. Cell tube formation analysis of endothelial cells that were treated with the same methods as those used in Fig. 2A. D. Cell proliferation rate analysis in infected ECs in the presence of FOXO3A siRNA or FOXO3A overexpressing lentivirus vector at 72hpi. E. Cell migration at 24 hours using the same methods as those used in Fig. 2D. F. Matrigel tube formation analysis using the same methods as those used in Fig. 2D. Data represents the mean value of three independent experiments, presented as Means±SD. <b>*</b>P and <b>^#</b>P<0.05. hpi, hour post infection; RSV, resveratrol. FOXO3A, foxo3a lentivirus vector; NC, normal control vector.