Analytical ultracentrifugation analysis of <i>pfi</i>T binding to recombinant HLA-DR1/HA complex.

<p>(<b>A</b>) Sedimentation coefficient distributions of <i>pfi</i>T alone at 3.6 µM concentration. (<b>B</b>) Sedimentation equilibrium analysis of <i>pfi</i>T. Representative absorbance distributions were shown for sedimentation equilibrium of <i>pfi</i>T at 18 µM (left panel) and 9 µM (right panel) at 20°C at rotor speeds of 20,000 rpm (black solid square), 25,000 rpm (red solid circle), and 30,000 rpm (green solid triangle). Distributions were analyzed as part of a global fitting to the absorbance data at multiple loading concentrations and multiple speeds. <i>Solid lines</i> are the global best-fit distributions using a reversible monomer and dimer model with SEDPHAT <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003837#ppat.1003837-Vistica1" target="_blank">[27]</a>. (<b>C</b>) Sedimentation equilibrium analysis of <i>pfi</i>T binding to the HLA-DR1/HA complex. Absorbance distributions were shown for sedimentation equilibrium of the <i>pfi</i>T-DR1 complex at different molar ratios at 20°C at rotor speeds of 20,000 rpm (black solid square), 25,000 rpm (red solid circle), and 30,000 rpm (green solid triangle). Data were analyzed as in (<b>B</b>).