Determination of the oligomeric state of Mtb Pck by analytical ultracentrifugation.

<p>(A) Sedimentation velocity experiment. Fitted data (upper panel) with residual plots (middle panel) representing the accuracy of the fit are shown together with the calculated continuous size distribution(s) of the sedimenting species (lower panel). (B) Equilibrium sedimentation distribution of Pck at 10–12–14–16–18–20–22,000 rpm. The upper panel shows absorbance data with fitted curves (non-interacting discrete species model, lines); the lower panel shows residuals derived from the fitted data.