Analytical ultracentrifugation of <i>At</i>-DHDPS2 and <i>At</i>-DHDPR2.

<p>Sedimentation velocity analysis of <i>At</i>-DHDPS2 and <i>At</i>-DHDPR2. A) Continuous sedimentation coefficient distribution [<i>(c)s</i>] analysis of <i>At-</i>DHDPS2 at a concentration of 0.75 mg.mL⁻¹ (black line). The RMSD and Runs Test Z (RTZ) scores for the fit were 0.008 and 3.2 respectively. B) <i>(c)s</i> analysis of <i>At-</i>DHDPR2 at concentrations of 0.1 mg.mL⁻¹ (black line; RMSD = 0.009, RTZ = 2.4), 0.2 mg.mL⁻¹ (red line; RMSD = 0.010, RTZ = 2.0), 0.4 mg.mL⁻¹ (green line; RMSD = 0.014, RTZ = 8.6) 0.8 mg.mL⁻¹ (pink line; RMSD = 0.013, RTZ = 4.9) and 1.6 mg.mL⁻¹ (blue line; RMSD = 0.015, RTZ = 7.4). Radial absorbance data for the three lower protein concentrations were acquired at a different wavelength to those of the two highest protein concentrations, and the <i>c(s)</i> distributions were scaled accordingly. Residuals for the fits are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040318#pone.0040318.s007" target="_blank">Figure S7</a>.