Characterization of <i>in vivo</i> biofilms of mutant strains of <i>L. reuteri</i> 100-23C.

<p>(A) Quantification of biofilm density (relative to biofilm of wild-type strain 100-23C) by confocal microscopy and cell counts in forestomach contents of <i>L. reuteri</i> mutants two days after gavage with a single dose of ∼10⁷ cells. ANOVA with Dunnett's multiple comparison test, *, <i>p</i><0.05; **, <i>p</i><0.01; ***, <i>p</i><0.001). Confocal micrographs of forestomach tissue from mice colonized for two days with (B) wild type, (C) <i>secA2</i> mutant, (D) <i>lysM3</i> mutant, and (E) <i>lr70902</i> mutant.