Characterization of the <i>in vitro</i> biofilm formation phenotype of the <i>cbk1</i>Δ <i>bcr1EE</i> double mutant.
- Pilar Gutiérrez-Escribano (323494)
- Ute Zeidler (244401)
- M. Belén Suárez (323495)
- Sophie Bachellier-Bassi (132285)
- Andrés Clemente-Blanco (323496)
- Julie Bonhomme (323497)
- Carlos R. Vázquez de Aldana (237032)
- Christophe d'Enfert (275077)
- Jaime Correa-Bordes (323498)
Publication date
February 2013
DOIAbstract
<p>(A) Quantification of adherence ability to plastic slides of wild-type (JC1089), <i>cbk1</i>Δ <i>BCR1</i> (JC1082), <i>cbk1</i>Δ <i>bcr1AA</i> (JC1096) and <i>cbk1</i>Δ <i>bcr1EE</i> (JC1099) cells. (B) The same strains were incubated during 40 hours in microfermentors and biofilm dry mass was determined. (C) CSLM images of biofilms induced using silicone squares after calcofluor white staining. (D) Quantitative RT-PCR measurements of <i>ALS3</i> and <i>ALS1</i> transcription levels in the wild-type, <i>cbk1</i>Δ <i>BCR1</i> and <i>cbk1</i>Δ <i>bcr1EE</i> strains normalized using <i>ADE2</i>.</p
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