Validation of microarray analysis by quantitative RT-PCR on tumor tissue samples.

<p>Data were first normalized to the expression of Arpc1 and Atp5b housekeeping genes. The fold changes (±SEM) in each gene expression was calculated using the mean expression in treated (n = 6 for microarray and n = 8 to 9 for RT-qPCR) versus untreated (n = 5 for microarray and n = 7 to 10 for RT-qPCR) tumors 6 h after MRT. Fold changes are indicated below the histograms (several fold changes were available for microarray because of the presence of several probesets). All tested genes presented a significant difference between treated and untreated tissues (p<0.05; permutation t-test).