ELISA analysis of binding of the monomeric and pentameric V<sub>H</sub>Hs to <i>C. jejuni</i> surface antigens.
- Ali Riazi (504588)
- Philippa C. R. Strong (504589)
- Russell Coleman (236828)
- Wangxue Chen (317694)
- Tomoko Hirama (194387)
- Henk van Faassen (279186)
- Matthew Henry (123102)
- Susan M. Logan (504590)
- Christine M. Szymanski (236840)
- Roger MacKenzie (194396)
- Mehdi Arbabi Ghahroudi (504591)
Publication date
December 2013
DOIAbstract
<p>Various concentrations (ranging from 10 to 0.0001 µg/ml) of the monomeric (FlagV1M) and pentameric (FlagV1P) forms of a flagella-specific V<sub>H</sub>H were used in ELISA. Absorbance data were normalized to represent equal amounts of the monomeric and pentameric V<sub>H</sub>H formats. Solid squares represent pentabody and open squares monomeric V<sub>H</sub>H. A representative image of Coomassie stained purified monomer and pentamer V<sub>H</sub>H is shown above the ELISA graphs.</p
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