<p>Ten selected phages named phages 1 to 10 were incubated with the H5N1 viruses in ELISA plates to test their binding activities to the viruses as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0023058#s2" target="_blank">Materials and methods</a>. The experiment was performed in triplicate. The individual phage and controls are indicated in the x axis. The controls 1–6 are phage library coating group, phage-free group, secondary antibody-free group, porcine transmissible gastroenteritis virus coating group, avian infectious bronchitis virus coating group, blocking buffer group and virus dilution solution coating group, respectively. The OD<sub>405</sub> values of tested individual phage and the OD value...
<p>(A) Titres of input and output phage populations throughout library panning. Pfu = phage-forming ...
a<p>Ten selected phages (phages 1 to 10) were subjected to phage DNA extraction and PCR. The deduced...
<p>(A) ELISA showing reactivity of purified antibodies at 30 µg/ml to HA antigen standard A/Californ...
<p>Twelve selected phages named phages 1 to 12 were incubated with the BRV in ELISA plates to test t...
<p>Serially diluted H5N1 viruses were used as coating antigens followed by successive incubation wit...
<p>Phages (1–3) harboring specific peptides recognizing the H5N1 viruses were incubated with the duc...
<p>The H5N1 viruses or H9N2 viruses were diluted in 0.1 M NaHCO<sub>3</sub> (pH8.6) to a final conce...
<p>(A) Polyclonal phage ELISA. The progress of selecting phage-V<sub>H</sub>Hs specific to α–Cbtx ov...
<p>Control and selected phage names are shown on the <i>z</i> axis, the OD<sub>490</sub> values of i...
<p>(A) after surface biopanning round 3 (B) after surface biopanning round 4A and (C) after solution...
<p>After three rounds of biopanning, 15 selected phage clones were detected by mAb 2D10 or an anti-p...
<p>Eight phage clones selected after three rounds of biopanning were added to the microplate wells (...
(A) Malaysian strains (B) WHO reference strains. Three negative controls (red) were included: medium...
<p>The relative binding affinity of single phage clones from mirror image phage display (clone numbe...
<p>Nine phage clones with different peptide sequences were used for the phage ELISA analysis. Phage ...
<p>(A) Titres of input and output phage populations throughout library panning. Pfu = phage-forming ...
a<p>Ten selected phages (phages 1 to 10) were subjected to phage DNA extraction and PCR. The deduced...
<p>(A) ELISA showing reactivity of purified antibodies at 30 µg/ml to HA antigen standard A/Californ...
<p>Twelve selected phages named phages 1 to 12 were incubated with the BRV in ELISA plates to test t...
<p>Serially diluted H5N1 viruses were used as coating antigens followed by successive incubation wit...
<p>Phages (1–3) harboring specific peptides recognizing the H5N1 viruses were incubated with the duc...
<p>The H5N1 viruses or H9N2 viruses were diluted in 0.1 M NaHCO<sub>3</sub> (pH8.6) to a final conce...
<p>(A) Polyclonal phage ELISA. The progress of selecting phage-V<sub>H</sub>Hs specific to α–Cbtx ov...
<p>Control and selected phage names are shown on the <i>z</i> axis, the OD<sub>490</sub> values of i...
<p>(A) after surface biopanning round 3 (B) after surface biopanning round 4A and (C) after solution...
<p>After three rounds of biopanning, 15 selected phage clones were detected by mAb 2D10 or an anti-p...
<p>Eight phage clones selected after three rounds of biopanning were added to the microplate wells (...
(A) Malaysian strains (B) WHO reference strains. Three negative controls (red) were included: medium...
<p>The relative binding affinity of single phage clones from mirror image phage display (clone numbe...
<p>Nine phage clones with different peptide sequences were used for the phage ELISA analysis. Phage ...
<p>(A) Titres of input and output phage populations throughout library panning. Pfu = phage-forming ...
a<p>Ten selected phages (phages 1 to 10) were subjected to phage DNA extraction and PCR. The deduced...
<p>(A) ELISA showing reactivity of purified antibodies at 30 µg/ml to HA antigen standard A/Californ...