Detection of the selected phages for antibody binding by Phage ELISA.

<p>Eight phage clones selected after three rounds of biopanning were added to the microplate wells (10¹⁰ pfu/100 µL/well) coated with the mAb A9E8 or anti-porcine IFN-γ mAb (negative control) (100 ng/well ), and incubated for 1 h at room temperature. Bound phages were subjected to reaction with horseradish peroxidase (HRP)-conjugated anti-M13 antibody, followed by color development with substrate solution containing o-phenylenediamine (OPD). Three independent assays were performed for each selected phage.