SPR analysis of the binding of the monomeric and pentameric V_HHs to antigen.

<p>Sensorgram overlays (A and B) represent the binding of 28, 42, 56, 70, 140 and 190 nM of FlagV1M and FlagV6M, respectively, to 700 RUs of biotinylated flagellin captured on an SA sensorchip. Black dots are the data points and the red curves are the fitting of the data to a 1∶1 interaction model. (C) The sensograms of FlagV1M (in red) and FlagV1P (in blue) were overlaid to show that a slower off-rate is obtained as a result of pentamerization indicating an increase in the functional affinity (avidity) of FlagV1P.