Molecular interaction network results from the literature-based Cognoscente knowledgebase where several genes with important roles in cardiac development were queried: Nkx2-5, Gata4, Gata5, Tbx1, Tbx5, Pitx2 and MEF2c (red boxes).

<p>Red arrows point from the TFs (proteins) to their targets (DNA), black solid lines represent protein-protein interactions and red dashed lines represent genetic interactions. Stacked boxes (which sometimes repeat the same gene name) in the figure show orthologs of genes in organisms where the ortholog has a documented interaction. Node colors indicate different model organisms as defined in the Taxa Key. Here, one example of the complicated transcriptional regulation is that Foxh1 transcription factor physically and functionally interacts with Nkx2-5 transcription factor to regulate the expression of Mef2c gene (inset region).