Interactome networks of select pathways identified as GR responsive by Ingenuity Pathway Analysis software.

<p>The IPA software organized and classified genes to identify important networks that were modulated by GR knockdown. These interactome networks detail the regulatory connections between genes as detailed by the connective arrows. The most strongly affected process was nervous system development at both 24 hpf (A) and 36 hpf (B). Other interesting pathways are DNA replication and energy production at 24 hpf (C), cardiovascular development at 36 hpf (D) and developmental disorders at 36 hpf (E). These final 3 pathways each involve a gene that were quantified by qPCR (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0080726#pone-0080726-g004" target="_blank">Figure 4</a>): <i>f5</i> (C, E), and <i>pomca</i> (D). Single-way arrows indicate one gene regulating another, two-sided arrows indicate co-regulation, looped arrows indicate self-regulation. The shape of each member of the network indicates its cellular location (according to IPA software classifications): Extracellular (diamond); plasma membrane (hexagon); cytosol (square); nucleus (circle); unknown (triangle). The color of each member of the network indicates its mean fold change range: >2 (dark green); 1-2 (light green); unchanged (grey); 0.5-1 (light red), <0.5 (dark red).