There were different level of Cav-1 expression in all five HCC cell lines and forced expression of Cav-1 leaded to up-regulation of cell migration and invasion and EMT phenotype of Huh7 cells.

<p>(A) The Cav-1 expression was different in five kinds of HCC cell lines; (B) Cav-1 expression in Huh7 cells was confirmed to be increased significantly after transfected with Cav-1 expressing plasmid by both qRT-PCR and Western immunoblotting; (C) As assessed by the wound healing assay, cell migration rate was increased in Huh7 cells by enforced expression of Cav-1 at both 24 and 48hours; (D) As assessed by Millicell invasion chamber assay, invasion capacity of Huh7 cells was increased apparently after overexpression of Cav-1; (E) After overexpression of Cav-1, E-cadherin expression was repressed and expression of mesenchymal markers including N-cadherin, Fibronectin and Vimentin was enhanced clearly.