Comparison of immune cell yield and distribution in endocervical cytobrush (CB) and ectocervical biopsy samples.

<p>(<b>A</b>) Representative gates for identification of CD45⁺ leukocytes from cytobrush and biopsy samples. (<b>B</b>) Numbers of CD45⁺ leukocytes, CD4⁺ T cells, CD8⁺ T cells, CD19⁺ B cells, CD14⁺ macrophages, and CD19^neg/HLA-DQ⁺ DC enumerated from cytobrush and biopsy samples. Cell numbers were log₁₀ transformed, and plotted per site (Chicago, red dots; Nairobi, blue dots; Seattle, black dots). Horizontal bars indicate median values for each site. (<b>C</b>) Percentage contribution of each cell subset, as well as of cells that do not fit one of the described populations (“unknown”), to the total CD45⁺ population from cytobrush and biopsy samples. Data are averaged across sites. P values are listed in the text, and median and IQR in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0085675#pone-0085675-t002" target="_blank">Table 2</a>.