Involvement of DSCC1 in CRC cell proliferation.

<p>(A) HCT116 cells were transfected with control (Mock and EGFP) and DSCC1 shRNAs for 48 h using Nucleofector kit, and western blot analysis was performed. Expression of β-actin served as a control. (B) Viability of cells transfected with shRNAs was measured by WST-8 assay. The data represents mean ± SD from three independent transfections. P values were calculated with the Dunnett's test for multiple comparisons to shEGFP-transfected cells. (C) Overexpression of DSCC1 in SW480 cells was confirmed by western blotting using anti-DSCC1 antibody. Equivalent number of three mock and three DSCC1 cells was plated in 96-well plates, and cell proliferation assays were performed at the indicated time points. The data represents mean ± SD from five experiments. A significant difference between mock and DSCC1 cells was determined by two-way repeated measures ANOVA.