Reduction of DmRecQ4 in S2 cells interferes with cell proliferation and DNA synthesis.

<p>A) Total extracts of S2 cells which had been mock depleted (control) or depleted of DmRecQ4 by dsRNA from the N terminal (NRecQ4) or C terminal (CRecQ4) region DmRecQ4 were analysed by western blotting with affinity purified anti-DmRecQ4 antibody. In each case extracts from 2.5×10 5 cells were loaded. The DmRecQ4 specific band is shown in the top panel and the bottom panel shows the tubulin control. B) Equal numbers of cells were treated with dsRNA against 2 different regions DmRecQ4 (N terminal – NRecQ4 and C terminal – CRecQ4), control dsRNA (TTC4) or left untreated (no RNA) and the proliferation of the cells was measured by performing a cell count on days 3,4, 5 and 6. C) Untreated cells and Cells treated with dsRNA against 2 different regions DmRecQ4 (N terminal – NRecQ4 and C terminal – CRecQ4) or against a control dsRNA were analysed for their cell cycle distribution using FACS analysis on days 3, 4, 5 and 6. D) Untreated cells and cells which had been exposed to dsRNA corresponding the N terminus of DmRecQ4 or control dsRNA were analysed for BrdU incorporation by dot blot titration 3 days after addition of dsRNA as described in the materials and methods.