MKT-077 potentiates 17-AAG induced HCC cells apoptosis.

<p>(A) The indicated cell lines were treated with MKT-077 (MKT) or 17-AAG alone or in combination at indicated dosages for 24 hours. Cell viability was qualified using the Cell Counting Kit-8 (Dojindo Laboratories). * denotes <i>P</i> < 0.05 comparing 17-AAG (1 <i>µ</i>M or 5 <i>µ</i>M) to MKT-077 (3 <i>µ</i>g/mL) +17-AAG (1 <i>µ</i>M or 5 <i>µ</i>M). (B) Increased cell apoptosis following combined treatment. Bel-7402, HuH7 and Hep3B cells treated as described above were subjected to Annexin-V and PI staining and cell apoptosis was quantified by FACS. * denotes <i>P</i> < 0.05 comparing 17-AAG (1 <i>µ</i>M or 5 <i>µ</i>M) to MKT-077 (3 <i>µ</i>g/mL) +17-AAG (1 <i>µ</i>M or 5 <i>µ</i>M). (C) Levels of cleaved PARP, GRP75, Akt, phospho-Akt^Ser473, phosphor-Akt^Thr308, p53, phospho-p53^Ser15, and phospho-p53^Ser37 were detected by Western Blotting analysis. GAPDH served as a loading control.