Effects of different inhibitory agents on 7KCh-induced cell death.

<p>ARPE19 cells were treated with 6-15 µM 7KCh for 24 hr and the cell viability was measured by determining cellular dehydrogenase activity. <b>(a)</b> Viability measurements with and without 10 µM CLI095 (mean ± s.d., <i>n</i> = 4). <b>(b)</b> Viability measurements with and without 50 µg/ml LPS (mean ± s.d., <i>n</i> = 3). <b>(c)</b> Viability measurements with and without 1 µM sterculic acid (mean ± s.d., <i>n</i> = 4). <b>(d)</b> Viability measurements with and without 5 µM AG1478 (mean ± s.d., <i>n</i> = 3). <b>(e)</b> Viability measurements with and without 10 µM LY294002 (mean ± s.d., <i>n</i> = 3). <b>(f)</b> Viability measurements with and without MKP2 overexpression (mean ± s.d., <i>n</i> = 4). CLI095, LPS, SA, and AG1478 reduced 7KCh-induced cell death while LY294002 and MKP2 overexpression had no effect. *<i>p</i><0.05, two-tailed Student's t-test.