Stable knockdown of JTB in HCC cells decreases cell apoptosis.

<p>HepG2 cells were treated with 0.6 mM H₂O₂ for 8 h. (A) The percentage of apoptotic cells in HepG2 cells. (B) Quantification of apoptosis cells in different HepG2 cells. The percentage of apoptotic cells was determined using cyto-fluorimetric analysis with Annexin-V and PI labeling. The data were obtained from three analyses (*<i>P</i><0.05). (C and D) The level of the Bcl-XL mRNA in HepG2 cells as measured using qRT-PCR. The results represent the mean of three amplifications and were calculated using the 2 exp^{(−△△Ct)} formula. β-actin mRNA in HepG2 cells was used as a control (*<i>P</i><0.05). (E) Western blot analysis of the cytosolic and mitochondrial cytochrome c levels in HepG2 cells induced by H₂O₂ after 8h. (F) Uncleaved (p47) and activated caspase-9 (p37) were detected by western blot analysis in HepG2 cells induced by H₂O₂ after 8 h.