PtdIns(4,5)P<sub>2</sub> is redistributed in response to PC(<i>O</i>-16:0/2:0).
- Michael A. Kennedy (230327)
- Kenneth Gable (514060)
- Karolina Niewola-Staszkowska (514061)
- Susana Abreu (514062)
- Anne Johnston (203659)
- Linda J. Harris (203661)
- Fulvio Reggiori (90842)
- Robbie Loewith (268205)
- Teresa Dunn (514063)
- Steffany A. L. Bennett (230346)
- Kristin Baetz (203062)
Publication date
January 2014
DOIAbstract
<p>Wild type (WT) cells (YPH500) expressing (<b>A</b>) GFP-2×PH<sup>PLCδ</sup> (PtdIns(4,5)P<sub>2</sub>) (<b>B</b>) GFP-PH<sup>Fapp</sup> (PtdIns(4)P) or (<b>C</b>) GFP-FYVE<sup>EEA1</sup> (PtdIns(3)P) were treated with either vehicle (EtOH) or PC(<i>O</i>-16:0/2:0) (20 µM, 15 min) and localization of the GFP probe quantified. The percentage of cells displaying a redistribution of the fluorescent reporter is reported in the inset of the figure.</p
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