Localization of PtdIns(4)P, PtdIns(4,5)P2 or PtdIns(3,4,5)P3 in PC12 cells.
- Jae-Sook Park (371787)
- Simon Halegoua (730688)
- Shosei Kishida (730689)
- Aaron M. Neiman (94539)
Publication date
April 2015
DOIAbstract
<p>(A) PC12 cells were transiently transfected with GFP-OSBP-PH, PLCδ1-PH-GFP or Akt-PH-GFP to visualize PtdIns(4)P, PtdIns(4,5)P<sub>2</sub> or PtdIns(3,4,5)P<sub>3</sub>, respectively. (B) PC12 cells were transfected with GFP-OSBP-PH to visualize PtdIns(4)P, fixed and stained with antibodies to the <i>cis</i>-Golgi marker GM130 and with DAPI. Representative images are shown. At least 15 cells were scored for each transfection. Scale bars = 5μm.</p
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