The GdmCl denaturation profiles of the EcRDBD mutants.

<p>The chemical denaturation experiments were conducted at 20°C, at protein concentrations of 2.5 µM, and respective denaturation curves were recorded using fluorescence measurement. Further details are given under <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0086052#s4" target="_blank"><i>Materials and Methods</i></a>. One of the three representative profiles for this experiment is shown. Panel A: filled circles – EcRDBD_WT (WT), asterisks – L16R, filled triangles – M49W, open diamonds – L58F, open triangles – C61A, open circles – V64E, x symbols – V64M, filled squares – UspDBD. Panel B: filled circles – EcRDBD_WT (WT), open diamonds – L16R/M49W/L58F, open circles – L16R/L58F, filled triangles – M49W/L58F, asterisks – L16R/M49W, filled squares – UspDBD.