Graphical presentation of guanidine hydrochloride (GdmCl) half concentration (C1/2), melting temperature (Tm) and the RosettaDesign scoring obtained for the EcRDBD mutants in comparison with the EcRDBDWT and UspDBD.

Histogram displaying the relative optical density (% OD) compared to the C. glutamicum wild type.

Lorenz C. Reimer (519603)
Jana Spura (519604)
Kerstin Schmidt-Hohagen (466334)
Dietmar Schomburg (19141)

February 2014

Measurements were done after 7 hours incubation in glucose minimal medium (MM). Mutants can be di...

High Resolution Melting derivative (dF/dT) profile for male and female samples.

Evguenia Alechine (191528)
Daniel Corach (294705)

May 2014

The figure shows each of the peaks for every amplified gene in the male sample (blue) and only AM...

Genotyping of G6PD mutations (G202A and A376G) by high resolution melting (HRM) assay.

Min Lin (17652)
Li Ye Yang (730776)
Dong De Xie (730777)
Jiang Tao Chen (730778)
Santiago-m Monte Nguba (730779)
Carlos Sala Ehapo (730780)
Xiao Fen Zhan (730781)
Juan Urbano Monsuy Eyi (730782)
Rocio Apicante Matesa (730783)
Maximo Miko Ondo Obono (730784)
Hui Yang (91136)
Hui Tian Yang (730785)
Ji Dong Cheng (730786)

April 2015

The arrowhead indicates different genotypes. A and C are temp-shift melting cures; ...

Guanidine hydrochloride (GdmCl) half concentration (C1/2) and melting temperature (Tm) obtained for the EcRDBDWT, its mutants and the UspDBD in chemical and thermal denaturation experiments in comparison with the RosettaDesign scoring.

Agnieszka Szamborska-Gbur (513806)
Grzegorz Rymarczyk (513807)
Marek Orłowski (31023)
Tomasz Kuzynowski (513808)
Michał Jakób (31021)
Agnieszka Dziedzic-Letka (513809)
Andrzej Górecki (513810)
Piotr Dobryszycki (513811)
Andrzej Ożyhar (31030)

January 2014

The GdmCl concentration was taken for 50% of the protein fraction unfolded each and melting tempe...

Chemical denaturation.

Francesca Malerba (161148)
Francesca Paoletti (161149)
Bruno Bruni Ercole (795980)
Serena Materazzi (144070)
Romina Nassini (144009)
Elisabetta Coppi (677153)
Riccardo Patacchini (144063)
Simona Capsoni (161132)
Doriano Lamba (179482)
Antonino Cattaneo (161159)

September 2015

Guanidinium Chloride (Gdm-Cl) denaturation profile of WT (blue), P61S (green), P61SR100E (red), R...

The GdmCl denaturation profiles of the EcRDBD mutants.

Agnieszka Szamborska-Gbur (513806)
Grzegorz Rymarczyk (513807)
Marek Orłowski (31023)
Tomasz Kuzynowski (513808)
Michał Jakób (31021)
Agnieszka Dziedzic-Letka (513809)
Andrzej Górecki (513810)
Piotr Dobryszycki (513811)
Andrzej Ożyhar (31030)

January 2014

The chemical denaturation experiments were conducted at 20°C, at protein concentrations of 2.5 µM...

Stability studies of GSTM2-2 and GST-C variants measured by circular dichroism (CD) and differential scanning fluorimetry.

Melanie J. Morris (354284)
Dan Liu (108669)
Llara M. Weaver (354285)
Philip G. Board (202313)
Marco G. Casarotto (202318)

February 2013

(a) Normalised molecular ellipticity at 222 nm as a function of guanidine ...

Comparison of fold changes in off-rate for gD as a function of sub-type (type-1 vs. type-2).

Tina M. Cairns (4110190)
Noah T. Ditto (4110187)
Huan Lou (203332)
Benjamin D. Brooks (714084)
Doina Atanasiu (631116)
Roselyn J. Eisenberg (203337)
Gary H. Cohen (203335)

June 2017

Fold change was calculated from ratio of gD2 off-rate over the gD1 off-rate. gD1 vs. gD2 was test...

High-resolution melting analysis.

Asraa Faris Aldoghachi (6260573)
Yin Sim Tor (750441)
Siti Zubaidah Redzun (6260576)
Khairul Aiman Bin Lokman (6260579)
Nurul Asyikin Abdul Razaq (6260582)
Aishah Farhana Shahbudin (6260585)
Ibrahim Mohamed Badamasi (6260588)
Pike-See Cheah (5279171)
Johnson Stanslas (6260591)
Abhi Veerakumarasivam (17425)
Rozita Rosli (3508277)
Normala Ibrahim (4875907)
Munn Sann Lye (6029600)
King-Hwa Ling (245535)

January 2019

A representative result of the normalized melting curves for (A) each genotype G/G, A/A and G/A BDNF...

Thermodynamic parameters characterising the guanidine-induced unfolding transition of GST-C and the mutants F157A and Y160A.

Melanie J. Morris (354284)
Dan Liu (108669)
Llara M. Weaver (354285)
Philip G. Board (202313)
Marco G. Casarotto (202318)

February 2013

Measurements were performed by monitoring the molar ellipticity at ...

Unfolding of WT and mutant CKs in GdnHCl solution.

Chang Li (133800)
Qian Zhang (49333)
Wei-Jiang Hu (133802)
Hang Mu (133804)
Zong Lin (133807)
Long Ma (133810)
Yong-Doo Park (133813)
Hai-Meng Zhou (133815)

September 2012

The activities at different concentrations of GdnHCl are plotted in (A). The transition of the pr...

A: Secondary structural features of HGDC and its mutants.

Venkata Pulla Rao Vendra (292388)
Garima Agarwal (227131)
Sushil Chandani (292389)
Venu Talla (442303)
Narayanaswamy Srinivasan (85259)
Dorairajan Balasubramanian (292391)

August 2013

Mean residue molar ellipticities, in degrees, of A (Blue): G165fs; B (Violet): L45PL54P; C (Maroo...

Comparison of the average RMSD values in the mutant regions (within 5Å distance from the disulfide bonds) between wild-type BglTM and the mutants.

Chengtuo Niu (1592089)
Linjiang Zhu (1592083)
Xin Xu (11989)
Qi Li (67548)

April 2016

Comparison of the average RMSD values in the mutant regions (within 5Å distance from the disulfid...

Scatter plot of G6PD activities of G6PD deficient and normal subjects from Trinity quantitative versus kinetics assays measured in U/g Hb.

Ari Winasti Satyagraha (699630)
Arkasha Sadhewa (699631)
Vanessa Baramuli (699632)
Rosalie Elvira (699633)
Chase Ridenour (699634)
Iqbal Elyazar (699635)
Rintis Noviyanti (106250)
Farah Novita Coutrier (699636)
Alida Roswita Harahap (699637)
J. Kevin Baird (120393)

March 2015

The different colours represented different variant (V in either normal, heterozygous and hemizyg...

Fluctuations of the WT and mutant residues during MD simulations.

Poornima Gajendrarao (391607)
Navaneethakrishnan Krishnamoorthy (391608)
Heba Sh Kassem (391609)
Sarah Moharem-Elgamal (391610)
Franco Cecchi (391611)
Iacopo Olivotto (391612)
Magdi H. Yacoub (75834)

March 2013

The average RMSF of the mutated residues for 10 ns are plotted in 2D bar graph. The bars in gray ...

Histogram displaying the relative optical density (% OD) compared to the C. glutamicum wild type.

Lorenz C. Reimer (519603)
Jana Spura (519604)
Kerstin Schmidt-Hohagen (466334)
Dietmar Schomburg (19141)

February 2014

Measurements were done after 7 hours incubation in glucose minimal medium (MM). Mutants can be di...

High Resolution Melting derivative (dF/dT) profile for male and female samples.

Evguenia Alechine (191528)
Daniel Corach (294705)

May 2014

The figure shows each of the peaks for every amplified gene in the male sample (blue) and only AM...

Genotyping of G6PD mutations (G202A and A376G) by high resolution melting (HRM) assay.

Min Lin (17652)
Li Ye Yang (730776)
Dong De Xie (730777)
Jiang Tao Chen (730778)
Santiago-m Monte Nguba (730779)
Carlos Sala Ehapo (730780)
Xiao Fen Zhan (730781)
Juan Urbano Monsuy Eyi (730782)
Rocio Apicante Matesa (730783)
Maximo Miko Ondo Obono (730784)
Hui Yang (91136)
Hui Tian Yang (730785)
Ji Dong Cheng (730786)

April 2015

The arrowhead indicates different genotypes. A and C are temp-shift melting cures; ...

Guanidine hydrochloride (GdmCl) half concentration (C1/2) and melting temperature (Tm) obtained for the EcRDBDWT, its mutants and the UspDBD in chemical and thermal denaturation experiments in comparison with the RosettaDesign scoring.

Agnieszka Szamborska-Gbur (513806)
Grzegorz Rymarczyk (513807)
Marek Orłowski (31023)
Tomasz Kuzynowski (513808)
Michał Jakób (31021)
Agnieszka Dziedzic-Letka (513809)
Andrzej Górecki (513810)
Piotr Dobryszycki (513811)
Andrzej Ożyhar (31030)

January 2014

The GdmCl concentration was taken for 50% of the protein fraction unfolded each and melting tempe...

Chemical denaturation.

Francesca Malerba (161148)
Francesca Paoletti (161149)
Bruno Bruni Ercole (795980)
Serena Materazzi (144070)
Romina Nassini (144009)
Elisabetta Coppi (677153)
Riccardo Patacchini (144063)
Simona Capsoni (161132)
Doriano Lamba (179482)
Antonino Cattaneo (161159)

September 2015

Guanidinium Chloride (Gdm-Cl) denaturation profile of WT (blue), P61S (green), P61SR100E (red), R...

The GdmCl denaturation profiles of the EcRDBD mutants.

Agnieszka Szamborska-Gbur (513806)
Grzegorz Rymarczyk (513807)
Marek Orłowski (31023)
Tomasz Kuzynowski (513808)
Michał Jakób (31021)
Agnieszka Dziedzic-Letka (513809)
Andrzej Górecki (513810)
Piotr Dobryszycki (513811)
Andrzej Ożyhar (31030)

January 2014

The chemical denaturation experiments were conducted at 20°C, at protein concentrations of 2.5 µM...

Stability studies of GSTM2-2 and GST-C variants measured by circular dichroism (CD) and differential scanning fluorimetry.

Melanie J. Morris (354284)
Dan Liu (108669)
Llara M. Weaver (354285)
Philip G. Board (202313)
Marco G. Casarotto (202318)

February 2013

(a) Normalised molecular ellipticity at 222 nm as a function of guanidine ...

Comparison of fold changes in off-rate for gD as a function of sub-type (type-1 vs. type-2).

Tina M. Cairns (4110190)
Noah T. Ditto (4110187)
Huan Lou (203332)
Benjamin D. Brooks (714084)
Doina Atanasiu (631116)
Roselyn J. Eisenberg (203337)
Gary H. Cohen (203335)

June 2017

Fold change was calculated from ratio of gD2 off-rate over the gD1 off-rate. gD1 vs. gD2 was test...

High-resolution melting analysis.

Asraa Faris Aldoghachi (6260573)
Yin Sim Tor (750441)
Siti Zubaidah Redzun (6260576)
Khairul Aiman Bin Lokman (6260579)
Nurul Asyikin Abdul Razaq (6260582)
Aishah Farhana Shahbudin (6260585)
Ibrahim Mohamed Badamasi (6260588)
Pike-See Cheah (5279171)
Johnson Stanslas (6260591)
Abhi Veerakumarasivam (17425)
Rozita Rosli (3508277)
Normala Ibrahim (4875907)
Munn Sann Lye (6029600)
King-Hwa Ling (245535)

January 2019

A representative result of the normalized melting curves for (A) each genotype G/G, A/A and G/A BDNF...

Thermodynamic parameters characterising the guanidine-induced unfolding transition of GST-C and the mutants F157A and Y160A.

Melanie J. Morris (354284)
Dan Liu (108669)
Llara M. Weaver (354285)
Philip G. Board (202313)
Marco G. Casarotto (202318)

February 2013

Measurements were performed by monitoring the molar ellipticity at ...

Unfolding of WT and mutant CKs in GdnHCl solution.

Chang Li (133800)
Qian Zhang (49333)
Wei-Jiang Hu (133802)
Hang Mu (133804)
Zong Lin (133807)
Long Ma (133810)
Yong-Doo Park (133813)
Hai-Meng Zhou (133815)

September 2012

The activities at different concentrations of GdnHCl are plotted in (A). The transition of the pr...

A: Secondary structural features of HGDC and its mutants.

Venkata Pulla Rao Vendra (292388)
Garima Agarwal (227131)
Sushil Chandani (292389)
Venu Talla (442303)
Narayanaswamy Srinivasan (85259)
Dorairajan Balasubramanian (292391)

August 2013

Mean residue molar ellipticities, in degrees, of A (Blue): G165fs; B (Violet): L45PL54P; C (Maroo...

Comparison of the average RMSD values in the mutant regions (within 5Å distance from the disulfide bonds) between wild-type BglTM and the mutants.

Chengtuo Niu (1592089)
Linjiang Zhu (1592083)
Xin Xu (11989)
Qi Li (67548)

April 2016

Comparison of the average RMSD values in the mutant regions (within 5Å distance from the disulfid...

Scatter plot of G6PD activities of G6PD deficient and normal subjects from Trinity quantitative versus kinetics assays measured in U/g Hb.

Ari Winasti Satyagraha (699630)
Arkasha Sadhewa (699631)
Vanessa Baramuli (699632)
Rosalie Elvira (699633)
Chase Ridenour (699634)
Iqbal Elyazar (699635)
Rintis Noviyanti (106250)
Farah Novita Coutrier (699636)
Alida Roswita Harahap (699637)
J. Kevin Baird (120393)

March 2015

The different colours represented different variant (V in either normal, heterozygous and hemizyg...

Fluctuations of the WT and mutant residues during MD simulations.

Poornima Gajendrarao (391607)
Navaneethakrishnan Krishnamoorthy (391608)
Heba Sh Kassem (391609)
Sarah Moharem-Elgamal (391610)
Franco Cecchi (391611)
Iacopo Olivotto (391612)
Magdi H. Yacoub (75834)

March 2013

The average RMSF of the mutated residues for 10 ns are plotted in 2D bar graph. The bars in gray ...

Histogram displaying the relative optical density (% OD) compared to the C. glutamicum wild type.

Lorenz C. Reimer (519603)
Jana Spura (519604)
Kerstin Schmidt-Hohagen (466334)
Dietmar Schomburg (19141)

February 2014

Measurements were done after 7 hours incubation in glucose minimal medium (MM). Mutants can be di...

High Resolution Melting derivative (dF/dT) profile for male and female samples.

Evguenia Alechine (191528)
Daniel Corach (294705)

May 2014

The figure shows each of the peaks for every amplified gene in the male sample (blue) and only AM...

Genotyping of G6PD mutations (G202A and A376G) by high resolution melting (HRM) assay.

Min Lin (17652)
Li Ye Yang (730776)
Dong De Xie (730777)
Jiang Tao Chen (730778)
Santiago-m Monte Nguba (730779)
Carlos Sala Ehapo (730780)
Xiao Fen Zhan (730781)
Juan Urbano Monsuy Eyi (730782)
Rocio Apicante Matesa (730783)
Maximo Miko Ondo Obono (730784)
Hui Yang (91136)
Hui Tian Yang (730785)
Ji Dong Cheng (730786)

April 2015

The arrowhead indicates different genotypes. A and C are temp-shift melting cures; ...

Graphical presentation of guanidine hydrochloride (GdmCl) half concentration (C<sub>1/2</sub>), melting temperature (T<sub>m</sub>) and the RosettaDesign scoring obtained for the EcRDBD mutants in comparison with the EcRDBD<sub>WT</sub> and UspDBD.

Abstract

Extracted data

Graphical presentation of guanidine hydrochloride (GdmCl) half concentration (C<sub>1/2</sub>), melting temperature (T<sub>m</sub>) and the RosettaDesign scoring obtained for the EcRDBD mutants in comparison with the EcRDBD<sub>WT</sub> and UspDBD.

Abstract

Extracted data

Related items

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