Denaturation of HMGB1 and HMGB1ΔC as a function of increasing Gdn.HCl concentration.

<p>A) The CM of HMGB1 (black circles) and HMGB1ΔC (red circles) at 5 μM was obtained for each [Gdn.HCl] using <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079572#eqn1" target="_blank">Equation 1</a>, as described in the Material and Methods Section. B) Trp fluorescence spectra were obtained and converted to degree of denaturation (α) according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079572#eqn2" target="_blank">Equation 2</a>. The resistance to unfolding can be analyzed by G_1/2, which reflects the concentration necessary to unfold 50% of the protein population and is detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0079572#pone-0079572-t001" target="_blank">Table 1</a>.