RAGE expression enhances cell-matrix adhesion.

<p>(<b>A</b>) Western blot analysis on rat lung lysate and R3/1 cells using three different antibodies recognizing RAGE extracellular (anti-RAGE N-term1 and anti-RAGE N-term2) or intracellular (anti-RAGE C-term) domains. Asterisk (*) indicates nonspecific bands. Forty µg of cell lysate were loaded and detection of GAPDH was used as loading control<b>.</b> (<b>B</b>) Western blot analysis on R3/1-pLXSN and R3/1-FL-RAGE cells using anti-RAGE N-term1 antibody. Forty µg of cell lysate were loaded and detection of GAPDH was used as loading control<b>.</b> (<b>C, D</b>) Cell-matrix adhesion assay. Adhesion of R3/1-pLXSN or R3/1-FL-RAGE cells onto culture dishes coated with 10 µg/ml ECM proteins. Adhesion to PBS, collagen I (Coll I), Fibronectin (FN), or Laminin (Lam) was assayed for 15 minutes (C) or 45 minutes (D). One representative experiment out of three is shown. Results from triplicate wells are displayed as means±SEM (ns, not significant; *, P<0.05; **, P<0.01).